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streptavidin conjugated horseradish peroxidase  (Vector Laboratories)


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    Vector Laboratories streptavidin conjugated horseradish peroxidase
    Streptavidin Conjugated Horseradish Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peroxidase+conjugated+streptavidin/DyLight+594+Streptavidin/pmc13133793-44-84-89
    Average 93 stars, based on 100 article reviews
    streptavidin conjugated horseradish peroxidase - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: A cloaked human stem-cell-derived neural graft capable of functional integration and immune evasion in rodent models.
    Article Snippet: Fluorescently labelled sections were cover-slipped with fluorescent mounting media (Dako); Cresyl and chromogenic labelled sections were dehydrated in alcohol and xylene and cover-slipped with DePeX mounting media (BDH Chemicals, UK) on gelatinized slides.

    Immunohistochemistry:

    Article Title: Apoptosis in kidney tissue of senior and geriatric cats with chronic kidney disease.
    Article Snippet: IHC for 8-OHdG and CD204 was performed as follows: (i) deparaffinization and rehydration; (ii) microwave heating using 10 mM citrate buffer (pH 6) at 700 W for 10 min; (iii) for CD204, treatment with 3% H2O2 for 30 min; (iv) washing with PBS; (v) blocking with 0.25% casein/PBS for 60 min; (vi) incubation with anti-8-OHdG mouse monoclonal antibody (1:800; MOG-020P; Japan Institute for the Control of Aging, Shizuoka, Japan) or anti-CD204 mouse monoclonal antibody (1:400; clone SRA-E5; Cosmo Bio, Tokyo, Japan) at 4°C overnight; (vii) washing with PBS; (viii) incubation with alkaline phosphatase polymer-conjugated universal antibody (Simple Stain AP [MULTI]; Nichirei Biosciences, Tokyo, Japan) for 8-OHdG or peroxidase-polymer-conjugated universal antibody (Simple Stain MAX-PO [MULTI]; Nichirei Biosciences) for CD204 for 30 min; (ix) washing with PBS; and (x) detection of immunoreactivity using Fast Red II Substrate Kit (Nichirei Biosciences) for 15 min for 8- OHdG, and DAB for 5 min for CD204. .. The IHC of 4-HNE and CD34 was performed with following reagents: anti-4HNE rabbit polyclonal antibody (1:50; 4°C overnight; ab46545; Abcam, Cambridge, UK), anti-CD34 goat polyclonal antibody (1:100; 4°C overnight; sc-7045; Santa Cruz Biotechnology, Dallas, TX, USA), biotinylated goat anti-rabbit IgG antibody (1:200; 30 min; Vector Laboratories, Newark, CA, USA), biotinylated rabbit antigoat IgG antibody (1:200; 30 min; Vector Laboratories), and peroxidase-conjugated streptavidin (ready-to-use; 30 min; Vector Laboratories). .. Sections were incubated with avidin and biotin blocking solutions (ready-to-use; 15 min each; Vector Laboratories) before incubation with the primary antibody.

    Incubation:

    Article Title: Nf2/FGFR1/AKT axis directs cranial neural crest-derived skull morphogenesis via collagen synthesis and trafficking.
    Article Snippet: Sections were then incubated with primary antibody in 10% normal donkey serum (NDS; D9663; Sigma-Aldrich) in PBSX at 4°C overnight (Supplemental Table 1). .. After rinses with PBS for 3 times, sections were incubated at 4°C for 24 hours with biotin-conjugated secondary antibody (BA-5000; Vector Laboratories) in 1% NDS in PBSX and then incubated at RT for 30 minutes with peroxidase-conjugated streptavidin in blocking solution (PK6200; Vector Laboratories). .. After 3 rinses in PBS, sections were preincubated in nickel-intensified DAB solution (SK4100; Vector Laboratories) without H2O2 at RT for 30 minutes followed by incubation with DAB solution containing 0.0003% H2O2.

    Article Title: Therapeutic potential of cell-permeable PEP-1-Srxn1 in mitigating oxidative and ischemic damage in the hippocampus.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: A cloaked human stem-cell-derived neural graft capable of functional integration and immune evasion in rodent models.
    Article Snippet: .. Secondary antibodies for i) direct detection included Alexa Fluor 488 or 647 (Jackson ImmunoResearch) or DyLight 550 (Abcam) conjugated donkey anti-goat, anti-rat, anti-mouse, anti-rabbit, and anti-sheep, and ii) chromogenic detection of antibody- DAB complex (biotin-conjugated donkey anti-rabbit, Jackson ImmunoResearch) were used at a dilution of 1:200, 2 h. For chromogenic staining, secondary antibodies incubation was followed by peroxidase conjugated streptavidin (2 hours, Vectastain ABC kit, Vector laboratories) and incubation with DAB (0.5 mg/ml, 2 min), which was precipitated by addition of 1% [w/v] H2O2. .. Fluorescently labelled sections were cover-slipped with fluorescent mounting media (Dako); Cresyl and chromogenic labelled sections were dehydrated in alcohol and xylene and cover-slipped with DePeX mounting media (BDH Chemicals, UK) on gelatinized slides.

    Article Title: Nf2/FGFR1/AKT axis directs cranial neural crest–derived skull morphogenesis via collagen synthesis and trafficking
    Article Snippet: Sections were then incubated with primary antibody in 10% normal donkey serum (NDS; D9663; Sigma-Aldrich) in PBSX at 4°C overnight ( ). .. After rinses with PBS for 3 times, sections were incubated at 4°C for 24 hours with biotin-conjugated secondary antibody (BA-5000; Vector Laboratories) in 1% NDS in PBSX and then incubated at RT for 30 minutes with peroxidase-conjugated streptavidin in blocking solution (PK6200; Vector Laboratories). .. After 3 rinses in PBS, sections were preincubated in nickel-intensified DAB solution (SK4100; Vector Laboratories) without H 2 O 2 at RT for 30 minutes followed by incubation with DAB solution containing 0.0003% H 2 O 2 .

    Article Title: Nf2-FAK signaling axis is critical for cranial bone ossification and regeneration.
    Article Snippet: Sections were then incubated with primary antibody in 10% normal donkey serum (D9663; Sigma Aldrich, St Louis, MO) in PBST at 4 °C overnight (Supplemental Table 1). .. After rinses with PBS for three time, sections were incubated at 4 °C for 24 h with biotin-conjugated secondary antibody (BA-5000; Vector Laboratories, Burlingame, CA) in 1% NDS in PBST, and then incubated at room temperature for 30min with peroxidase-conjugated streptavidin in blocking solution (PK6200; Vector Laboratories, Burlingame, CA). .. After three rinses in PBS, sections were preincubated in nickel-intensified DAB solution (SK4100; Vector Laboratories, Burlingame, CA) without H2O2 at room temperature for 30min followed by incubation with DAB solution containing 0.0003% H2O2.

    Article Title: Nf2-FAK signaling axis is critical for cranial bone ossification and regeneration
    Article Snippet: Sections were then incubated with primary antibody in 10% normal donkey serum (D9663; Sigma Aldrich, St Louis, MO) in PBST at 4 °C overnight (Supplemental Table ). .. After rinses with PBS for three time, sections were incubated at 4 °C for 24 h with biotin-conjugated secondary antibody (BA-5000; Vector Laboratories, Burlingame, CA) in 1% NDS in PBST, and then incubated at room temperature for 30 min with peroxidase-conjugated streptavidin in blocking solution (PK6200; Vector Laboratories, Burlingame, CA). .. After three rinses in PBS, sections were preincubated in nickel-intensified DAB solution (SK4100; Vector Laboratories, Burlingame, CA) without H 2 O 2 at room temperature for 30 min followed by incubation with DAB solution containing 0.0003% H 2 O 2 .

    Blocking Assay:

    Article Title: Nf2/FGFR1/AKT axis directs cranial neural crest-derived skull morphogenesis via collagen synthesis and trafficking.
    Article Snippet: Sections were then incubated with primary antibody in 10% normal donkey serum (NDS; D9663; Sigma-Aldrich) in PBSX at 4°C overnight (Supplemental Table 1). .. After rinses with PBS for 3 times, sections were incubated at 4°C for 24 hours with biotin-conjugated secondary antibody (BA-5000; Vector Laboratories) in 1% NDS in PBSX and then incubated at RT for 30 minutes with peroxidase-conjugated streptavidin in blocking solution (PK6200; Vector Laboratories). .. After 3 rinses in PBS, sections were preincubated in nickel-intensified DAB solution (SK4100; Vector Laboratories) without H2O2 at RT for 30 minutes followed by incubation with DAB solution containing 0.0003% H2O2.

    Article Title: Nf2/FGFR1/AKT axis directs cranial neural crest–derived skull morphogenesis via collagen synthesis and trafficking
    Article Snippet: Sections were then incubated with primary antibody in 10% normal donkey serum (NDS; D9663; Sigma-Aldrich) in PBSX at 4°C overnight ( ). .. After rinses with PBS for 3 times, sections were incubated at 4°C for 24 hours with biotin-conjugated secondary antibody (BA-5000; Vector Laboratories) in 1% NDS in PBSX and then incubated at RT for 30 minutes with peroxidase-conjugated streptavidin in blocking solution (PK6200; Vector Laboratories). .. After 3 rinses in PBS, sections were preincubated in nickel-intensified DAB solution (SK4100; Vector Laboratories) without H 2 O 2 at RT for 30 minutes followed by incubation with DAB solution containing 0.0003% H 2 O 2 .

    Article Title: Nf2-FAK signaling axis is critical for cranial bone ossification and regeneration.
    Article Snippet: Sections were then incubated with primary antibody in 10% normal donkey serum (D9663; Sigma Aldrich, St Louis, MO) in PBST at 4 °C overnight (Supplemental Table 1). .. After rinses with PBS for three time, sections were incubated at 4 °C for 24 h with biotin-conjugated secondary antibody (BA-5000; Vector Laboratories, Burlingame, CA) in 1% NDS in PBST, and then incubated at room temperature for 30min with peroxidase-conjugated streptavidin in blocking solution (PK6200; Vector Laboratories, Burlingame, CA). .. After three rinses in PBS, sections were preincubated in nickel-intensified DAB solution (SK4100; Vector Laboratories, Burlingame, CA) without H2O2 at room temperature for 30min followed by incubation with DAB solution containing 0.0003% H2O2.

    Article Title: Nf2-FAK signaling axis is critical for cranial bone ossification and regeneration
    Article Snippet: Sections were then incubated with primary antibody in 10% normal donkey serum (D9663; Sigma Aldrich, St Louis, MO) in PBST at 4 °C overnight (Supplemental Table ). .. After rinses with PBS for three time, sections were incubated at 4 °C for 24 h with biotin-conjugated secondary antibody (BA-5000; Vector Laboratories, Burlingame, CA) in 1% NDS in PBST, and then incubated at room temperature for 30 min with peroxidase-conjugated streptavidin in blocking solution (PK6200; Vector Laboratories, Burlingame, CA). .. After three rinses in PBS, sections were preincubated in nickel-intensified DAB solution (SK4100; Vector Laboratories, Burlingame, CA) without H 2 O 2 at room temperature for 30 min followed by incubation with DAB solution containing 0.0003% H 2 O 2 .

    Staining:

    Article Title: A cloaked human stem-cell-derived neural graft capable of functional integration and immune evasion in rodent models.
    Article Snippet: .. Secondary antibodies for i) direct detection included Alexa Fluor 488 or 647 (Jackson ImmunoResearch) or DyLight 550 (Abcam) conjugated donkey anti-goat, anti-rat, anti-mouse, anti-rabbit, and anti-sheep, and ii) chromogenic detection of antibody- DAB complex (biotin-conjugated donkey anti-rabbit, Jackson ImmunoResearch) were used at a dilution of 1:200, 2 h. For chromogenic staining, secondary antibodies incubation was followed by peroxidase conjugated streptavidin (2 hours, Vectastain ABC kit, Vector laboratories) and incubation with DAB (0.5 mg/ml, 2 min), which was precipitated by addition of 1% [w/v] H2O2. .. Fluorescently labelled sections were cover-slipped with fluorescent mounting media (Dako); Cresyl and chromogenic labelled sections were dehydrated in alcohol and xylene and cover-slipped with DePeX mounting media (BDH Chemicals, UK) on gelatinized slides.



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